EKSTRAK DAUN TEH HIJAU (CAMELLIA SINENSIS) SEBAGAI XANTIOKSIDAN ALAMI MINYAK KELAPA (COCOS NUCIFERA)
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Keywords:
Green tea, coconut oil, natural antioxidant, phenolic extraction
Abstract
Coconut oil can be used to fulfill the high demand for cooking oil in Indonesia. The quality of coconut oil can decrease because of oxidation. Oxidation can be prevented by adding natural antioxidant such as green tea. The objective of this research was to optimize the process of metabolite extraction with highest antioxidant activity. The solvent used for extraction were ethanol 96%, asetonitril 50% and acetone 25%. Green tea extract was analyzed quantitatively using Total Phenolic Compound (TPC) and 2,2-diphenyl-1-pichrylhydraziyl (DPPH). The crude extract from green tea from maceration using ethanol 96% gave the highest inhibition at about 90,8 ± 0,71%. Thin Layer Chromatography (TLC) analysis showed existence of catechin and myrecetin in the extract. As much as 0,01% was applied as natural antioxidant in coconut oil. The quality of coconut oil observed by iodine value, peroxide value, TBA value, colour and viscosity changed. The result showed that the addition of green tea extract can prevent oxidation rate with efficiency of 1,5.
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References
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Tensika, Marsetio, & Yudiastuti. 2007. Pengaruh Jenis Pelarut terhadap Aktivitas Antioksidan Ekstrak Kasar Isoflavon dari Ampas Tahu.
Tomsone, Lolita., Zanda Kruma., Ruta Galoburda. 2012. Comparison of Different Solvents and Extraction Methods for Isolation of Phenolic Compounds from Horseradish Roots (Armoracia rusticana). World Ac ademy of Science, Engineering and T echnology International Journal of B iological, Biomolecular, Agricultural, Food and B iotechnological Engineering Vol:6, No:4, 2012
Uzunalic, A.; M. Škerget; Ž. Knez, B. Weinreich, F. Otto & S. Grûner (2006). Ex traction of active in gredients from g reen tea (Camellia sinensis): Extraction efficiency of ma jor cat echins and caff eine. Food Chemistry, 96, 597—605
Widyasanti Asri, Da dan & Novriana. 2016. Akt ivitas Antioksidan Ekstrak The Putih dengan Metode DPPH. Fortech
Yates, R. A dan J.d. Caldwell. 1992. Adsorptive Capacity of Active Filter Aids for Used Cooking Oil. 69 (9); 894-897.
Anie Komayaharti, Dwi Paryanti. “Ekstrak Daun Sirih sebagai Antioksidan Pada Minyak Kelapa”. Jurusan Teknik Kimia Fakultas Teknik UNDIP, Semarang, 2009.
Augustyn, G.H. 2012. The Effect of Papaya Fruit (Carica papaya L.) Extract on Virgin Coconut Oil Quality. Jurnal Budidaya Pertanian 8: 55–60.
Ayucitra, A., Indraswati, N., Mulda yansari, V., Dengi, Y. K., F ransisco G., dan Yudha. A. 2011 ‘Potensi Senyawa Fenolik Bahan Alam sebagai Antioksidan alami Minyak Goreng Nabati’. Widya Tectic.Vol 10,0.1.
Erawati, 2012. Uji Aktivitas Antioksidan Ekstrak Daun Garciniadeadalanthera Pierre dengan Metode DPPH dan Identifikasi Golongan Senyawa Kimia dari Fraksi Paling Aktif. FMIPA Universitas Indonesia
Javanmardi, J., Stushnoff, C., Locke, E., and Vivanco, J.M., 2003, Antiox idant Activity and Total Phenolic Content of Iranian Ocimum Accessions, J. Food Chem., 83, 547-550.
Karouw, S., Suparmo, Hastuti, P. dan Utami, T. 2013. Sintesis ester metil ra ntai medium dari minyak kelapa dengan cara metanolisis kimiawi. Agritech 33(2): 182-188.
Ketaren, S. , 1986, Pen gantar Teknologi Minyak dan Lemak Pangan, Jakarta : Universitas Indonesia.
Ligor Magdalena, Olga Kornyšova, Audrius Maruška, and Bogusław Buszewski. 2008. Determination of Flavonoids in Tea and Rooibos Extracts by TLC and HPLC. Journal of planar chromatography. Vol 21, pp.355-360
Marten, B., Pfeuffer, M. dan Schrezenmeir, J. 2006. Medium-chain triglycerides : Review. International Dairy Journal 16: 1374-1382.
Mbata, T.I., Debiao, L.U. & Saikia, A., 2006. Antibacterial activity of the crude extract of Chinese green tea (Camellia sinensis) on Listeria monocytogenes. Afr. J. Biotechnol., 7(10): 1571-1573. Available at http://www.academicjournals.org/AJB. [Accessed on 9 April 2012].
Menteri Perindustrian Republik Indonesia. 2013. http://kemenperin.go.id/artikel/3987/SNI-Wajib-Minyak-Goreng- Diterapkan-2013, diakses pada 25 agustus 2016
Moechtar. 1990. “Farmasi Fisika Bagian Srtuktur Atom dan Molekul Zat Padat dan Mikromeretika”. Yogyakarta, Gajah Mada University Press.
Moehyi, S. 1992. Penyelenggaran Makanan Institusi Dan Jasa Boga. Jakarta : Bhatara
Mutiara, E. V, Sulistyowati, E, dan Pertiwi, H. T. 2009. Pengaruh Ekstrak Etanol Bekatul Padi (Oryza sativa L.) terhadap Penghambatan Ketengikan Minyak dibandingkan dengan Antioksidan Sintetik BHT (Butylated Hydroxy Toluene). Media Farmasi Indonesia Vol 4, No 1
Nielsen, S. S. 2009. Food Analysis Fourth Edition. NewYork : Springer.
Rezaeizadeh, A., ABZ Zuki, Abdollahi M., Y. M., Goh, M. M. Noordin, M. Hamid & TI Azmi, 2011, Determination of Antioxidant Activity in Methanolic and Chloroformic Extract of Momordica carantia, African J ournal of Biotechnology, 10 (24), 4932-4940.
Rindit Pambayun, Murdijati Gardjito, Slamet Sudarmadji, Kapti Rahayu Kuswanto. 2007. Phenolic content and antibacterial properties of various ex tracts of gambir (Uncaria gambir Roxb)
Salamah, Ummi. (2007). Hubung an Kualitas Minyak Goreng yang Digunakan Secara Berulang Terhadap Umur Simpan Keripik Sosis Ayam. http://repository.ipb.ac.id/handle/123456789/10454 Diakses 16 November 2012
Sudarmadji, S. 1997. Prosedur Analisis Untuk Bahan Makanan dan Pertanian. Edisi ke tiga, Liberty, Yokyakarta
Sulistyo, J, Nurdiana, H, Elizar. 2003. Pengembangan Kerja Sama Riset, Teknologi Produksi, dan Pemasaran Produk Hilir Teh. Prosiding ”Simposium Teh Nasional 2003”. Bandung : Pusat Penelitian Teh Kina Gambung.
Susilo. B, Asmandani, A., dan B. Sumardi. 2013. Pengolahan Limbah Daun Kelapa Sawit (Elaeis guieensis JACQ) dan Ampas Singkong Sebagai Alternatif Pakan Tambahan Untuk Ternak Ruminansia. Jurnal Keteknikan Pertanian Tropis. Vol. 1 No. 1 29-34.
Tensiska, C H anny Wijaya & Nuri Andarwulan. 2003. Aktivitas Anti oksidan Ekstrak Buah Andaliman (Zanthoxylum acanthopodium) dalam beb erapa Sistem Pangan dan Kestabilan Aktivitasnya terhadap Kondisi Suhu dan p H. Jurnal Teknol dan Industri Pangan, Vol XIV, no 1.
Tensika, Marsetio, & Yudiastuti. 2007. Pengaruh Jenis Pelarut terhadap Aktivitas Antioksidan Ekstrak Kasar Isoflavon dari Ampas Tahu.
Tomsone, Lolita., Zanda Kruma., Ruta Galoburda. 2012. Comparison of Different Solvents and Extraction Methods for Isolation of Phenolic Compounds from Horseradish Roots (Armoracia rusticana). World Ac ademy of Science, Engineering and T echnology International Journal of B iological, Biomolecular, Agricultural, Food and B iotechnological Engineering Vol:6, No:4, 2012
Uzunalic, A.; M. Škerget; Ž. Knez, B. Weinreich, F. Otto & S. Grûner (2006). Ex traction of active in gredients from g reen tea (Camellia sinensis): Extraction efficiency of ma jor cat echins and caff eine. Food Chemistry, 96, 597—605
Widyasanti Asri, Da dan & Novriana. 2016. Akt ivitas Antioksidan Ekstrak The Putih dengan Metode DPPH. Fortech
Yates, R. A dan J.d. Caldwell. 1992. Adsorptive Capacity of Active Filter Aids for Used Cooking Oil. 69 (9); 894-897.
Published
2018-03-01
Section
Bidang Teknobiologi (TB)
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