Multi Mycotoxins Analysis in Rice Using LC-MS/MS

Abstract

Multi mycotoxins analysis in this research has the purpose to develop method of analysis
for rice sample with a simple and fast preparation and novel technology using LC-MS/MS. Several blank
rice samples were spiked with standards of 8 classes of mycotoxins (Aflatoxin B1, Aflatoxin B2, Aflatoxin
G1, Aflatoxin G2, Deoxynivalenol, Zearalenon, T-2, and HT-2). Parameter of linearity, precision, recovery,
limit of detection and limit of quantification were obtained and calculated. The result shows that all the
validation parameters comply with criteria of method validation. Sonication method that was chosen to
extract analites and Solid Phase Extraction Column that used for sample clean up are successful methods
that have contribution for precise and accurate data. The Identification and quantification with LC-MS/
MS help to determine and distinguish each mycotoxin through chromatogram appearance, retention time
and a specific m/z.