FORMULASI DAN PENETAPAN KADAR NANOPARTIKEL KUERSETIN PRE DAN PASCA PROSES FREEZE DRYING DENGAN KRIOPROTEKTAN MALTODEKSTRIN-GLISERIN
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formulasi, nano partikel, kuersetin, freeze drying, krioprotektan, maltodekstrin, gliserin
Abstract
Abstrak—Pada penelitian ini dilakukan formulasi freeze drying nanopartikel kuersetin dengan Krioprotektan untuk menjaga stabilitas obat dan menggunakan HPLC untuk membandingkan kadar kuersetin blank dengan kadar kuersetin setelah freeze drying menggunakan cyroprotectan. Formula terpilih ditentukan berdasarkan sifat fisik (detailkan ukuran partikel, kandungan lembab, morfologi permukaan dengan SEM, bobot jenis, dll), dan kestabilan fisika-kimia yang paling optimal. Keberhasilan dalam meningkatkan karakteristik fisikokimia diduga menunjukkan peningkatan bioavailabilitas dan aktivitas senyawa gabungan untuk studi lebih lanjut. Penelitian ini dilakukan dengan tujuan untuk menentukan apakah formulasi nanokristal kuersetin dapat mempengaruhi ukuran partikel pada sampel dan untuk mengetahui apakah Formulasi nanokristal kuersetin dengan maltodektrin dan gliserin dapat menjaga kestabilitasan kimia sediaan. Hasil analisis menunjukkan kadar kuersetin pada sampel formulasi 1 yaitu 66,44% dengan bobot awal 103,5 mg bertambah menjadi 106,8 mg setelah freeze drying. Pada sampel formulasi 2 diperoleh kadar 95%, namun bobot kuersetin tetap berbobot 101,05 mg. Pada sampel formulasi 3 diperoleh kadar 78,83%, dengan bobot kuersetin yang bertambah dari 99,8 mg menjadi 110,5 mg. Artinya, replikasi formulasi nanopartikel kuersetin dengan gliserin mengandung kuersetin yang lebih besar dibandingkan dengan maltodektrin atau gabungan keduanya. Kedua senyawa yang digunakan dapat mempertahankan stabilitas kimia kuersetin yang diformulasi.
Kata kunci: formulasi, nano partikel, kuersetin, freeze drying, krioprotektan, maltodekstrin, gliserin
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References
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Aubry, J. M. (2013). CRF system and mood disorders. Journal of Chemical Neuroanatomy, 54, 20–24. https://doi.org/10.1016/j.jchemneu.2013.09.003
Carvalho, A. F., Sharma, M. S., Brunoni, A. R., Vieta, E., & Fava, G. A. (2016). The Safety, Tolerability and Risks Associated with the Use of Newer Generation Antidepressant Drugs: A Critical Review of the Literature. Psychotherapy and Psychosomatics, 85(5), 270–288. https://doi.org/10.1159/000447034
Chen, S., Tang, Y., Gao, Y., Nie, K., Wang, H., Su, H., Wang, Z., Lu, F., Huang, W., & Dong, H. (2022). Antidepressant Potential of Quercetin and its Glycoside Derivatives: A Comprehensive Review and Update. In Frontiers in Pharmacology (Vol. 13). Frontiers Media S.A. https://doi.org/10.3389/fphar.2022.865376
Christoph, R., Schmidt, B., Steinberner, U., Dilla, W., & Karinen, R. (2006). Glycerol. Ullmann’s Encyclopedia of Industrial Chemistry. https://doi.org/10.1002/14356007.A12_477.PUB2
Dams, R. I., Viana, M. B., Guilherme, A. A., Silva, C. M., dos Santos, A. B., Angenent, L. T., Santaella, S. T., & Leitão, R. C. (2018). Production of medium-chain carboxylic acids by anaerobic fermentation of glycerol using a bioaugmented open culture. Biomass and Bioenergy, 118, 1–7. https://doi.org/10.1016/J.BIOMBIOE.2018.07.023
Depressive disorder (depression). (n.d.). https://www.who.int/news-room/fact-sheets/detail/depression
D’mello, P. M., Joshi, U. J., Shetgiri, P. P., Dasgupta, T. K., & Darji, K. K. (n.d.). A Simple HPLC Method for Quantitation of Quercetin in Herbal Extracts. https://academic.oup.com/jaoac/article/94/1/100/5655334
Gerber, F., Krummen, M., Potgeter, H., Roth, A., Siffrin, C., & Spoendlin, C. (2004). Practical aspects of fast reversed-phase high-performance liquid chromatography using 3 μm particle packed columns and monolithic columns in pharmaceutical development and production working under current good manufacturing practice. Journal of Chromatography A, 1036(2), 127–133. https://doi.org/10.1016/J.CHROMA.2004.02.056
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Hofman, D. L., van Buul, V. J., & Brouns, F. J. P. H. (2016). Nutrition, Health, and Regulatory Aspects of Digestible Maltodextrins. Critical Reviews in Food Science and Nutrition, 56(12), 2091–2100. https://doi.org/10.1080/10408398.2014.940415
Izutsu, K. I. (2018). Applications of freezing and freeze-drying in pharmaceutical formulations. In Advances in Experimental Medicine and Biology (Vol. 1081, pp. 371–383). Springer New York LLC. https://doi.org/10.1007/978-981-13-1244-1_20
Jakubowska, E., Bielejewski, M., Milanowski, B., & Lulek, J. (2022). Freeze-drying of drug nanosuspension– study of formulation and processing factors for the optimization and characterization of redispersible cilostazol nanocrystals. Journal of Drug Delivery Science and Technology, 74. https://doi.org/10.1016/j.jddst.2022.103528
Kar, M., Chourasiya, Y., Maheshwari, R., & Tekade, R. K. (2019). Current Developments in Excipient Science: Implication of Quantitative Selection of Each Excipient in Product Development. Basic Fundamentals of Drug Delivery, 29–83. https://doi.org/10.1016/B978-0-12-817909-3.00002-9
Koop, S. (2016). VALIDATION OF COMPUTERIZED SYSTEMS Background information.
LaCourse, M. E., & LaCourse, W. R. (2017). General instrumentation in HPLC *. In Liquid Chromatography (pp. 417–429). Elsevier. https://doi.org/10.1016/B978-0-12-805393-5.00017-8
Malviya, R., Bansal, V., Pal, O., & Sharma, P. (2010). High performance liquid chromatography: A short review. Journal of Global Pharma Technology, 2, 22–26.
Pannu, A., Sharma, P. C., Thakur, V. K., & Goyal, R. K. (2021). Emerging role of flavonoids as the treatment of depression. In Biomolecules (Vol. 11, Issue 12). MDPI. https://doi.org/10.3390/biom11121825
Pradana, A. T., Nawatila, R., Alkindi, F. F., Darmayani, N. P. R., & Susanti, E. D. (2021). The effect of spray-drying temperature on Centella asiatica extract-β cyclodextrin-maltodextrin nanoparticle characteristics and stability. Pharmaciana, 11(3), 394. https://doi.org/10.12928/pharmaciana.v11i3.21534
Sahoo, N. G., Kakran, M., Shaal, L. A., Li, L., Müller, R. H., Pal, M., & Tan, L. P. (2011). Preparation and characterization of quercetin nanocrystals. Journal of Pharmaceutical Sciences, 100(6), 2379–2390. https://doi.org/10.1002/jps.22446
Ulusoy, H. G., & Sanlier, N. (2020). A minireview of quercetin: from its metabolism to possible mechanisms of its biological activities. In Critical Reviews in Food Science and Nutrition (Vol. 60, Issue 19, pp. 3290–3303). Bellwether Publishing, Ltd. https://doi.org/10.1080/10408398.2019.1683810
Wang, G. D., Mallet, F. P., Ricard, F., & Heng, J. Y. Y. (2012). Pharmaceutical nanocrystals. Current Opinion in Chemical Engineering, 1(2), 102–107. https://doi.org/10.1016/J.COCHE.2011.12.001
Wang, Y. S., Shen, C. Y., & Jiang, J. G. (2019). Antidepressant active ingredients from herbs and nutraceuticals used in TCM: pharmacological mechanisms and prospects for drug discovery. In Pharmacological Research (Vol. 150). Academic Press. https://doi.org/10.1016/j.phrs.2019.104520
United States Pharmaceutical Convention. (2020). USPNF2021: Validation of Compend Procedures <1225>: Issue 1. Available from: https://online.uspnf.com/uspnf
Liang, R., Xu, S., Shoemaker, C. F., Li, Y., Zhong, F., & Huang, Q. (2012). Physical and antimicrobial properties of peppermint oil nanoemulsions. Journal of Agricultural and Food Chemistry, 60(30), 7548-7555. DOI: https://doi.org/10.1021/jf301129k
Published
2025-05-31
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BIDANG FARMASI
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