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Citrus aurantifolia (lime) is the natural ingredients having potential activity as an antiseptic. This
study aimed to formulate a hand wash liquid soap with active ingredients lime juice and to evaluate its antiseptic
activity. Three different formulas (Formula I, Formula II, and Formula III, with a concentration of lime juice 20,
30, and 40%, respectively) were evaluated for their pH, visual appearance, density, and viscosity. Antiseptic activity
test was carried out using the replica method. The result showed that lime juice hand wash soap had a transparent,
homogen, pale yellow color, and lime odor. Viscosity and pH of the preparation did not meet the criteria
of SNI (National Standart of Indonesia). Moreover, the results showed that an increased of lime juice concentration
implied a significant decrease in viscosity and pH of the lime juice hand wash. Antiseptic activity test was indicated
by the percentage of bacterial reduction. The percentage of bacterial reduction for Formula I, II, and III
were 61.25, 68.89, and 89.06%, respectively. Statistical evaluation using one-way Anova (α = 0.05) followed by
LSD showed the percentage of bacterial reduction in three formulas were significantly different compared to
the control formula (percentage of bacterial reduction = 35.8%). However, when the result were compared
to the positive control (percentage of bacterial reduction = 71.79%), there was no significant difference.
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