Simultaneous HPLC Determination of Lidocaine Hydrochloride and Hexachlorophene in a Suppository Product

  • Cece Furwanti Graduate Program, Faculty of Pharmacy, University of Surabaya, Surabaya
  • Kusuma Hendrajaya Pharmaceutical Chemistry Department, Faculty of Pharmacy, University of Surabaya, Surabaya
  • Gunawan Indrayanto Graduate Program, Faculty of Pharmacy, University of Surabaya, Surabaya

Abstract

Antihemoroid® suppository has been produced commercially by PT. Kimia Farma, Indonesia. For QC purposes, a separated densitometric method for analysis of its active ingredients, lidocaine hydrochloride and hexachlorophene, was applied. The objective of this study was obtaining more efficient analysis method of LH and HC, therefore an HPLC procedure has been developed for the determination of both compounds simultaneously. AYMC-Triart C18 column was used with a gradient mobile phase consisting of acetonitrile and phosphate buffer 0.05 M (pH 6.0). Quantitative evaluation was performed at 220 nm. Method validation was performed according to the new methods of USP 41. Result showed that the HPLC method was simple, accurate, precise, and robust. The HPLC method can be applied in simultaneous determination of LH and HC in suppositories as a QC tool in the pharmaceutical industries.

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Published
2020-06-18
How to Cite
FURWANTI, Cece; HENDRAJAYA, Kusuma; INDRAYANTO, Gunawan. Simultaneous HPLC Determination of Lidocaine Hydrochloride and Hexachlorophene in a Suppository Product. MPI (Media Pharmaceutica Indonesiana), [S.l.], v. 3, n. 1, p. 27-36, june 2020. ISSN 2527-9017. Available at: <https://journal.ubaya.ac.id/index.php/MPI/article/view/2455>. Date accessed: 27 sep. 2020. doi: https://doi.org/10.24123/mpi.v3i1.2455.
Section
Original Research Articles