Rhizome Buds Disinfection for Preparation of Red Ginger (Zingiber officinale Roxb. var. rubrum Rosc.) In Vitro Culture

  • Popy Hartatie Hardjo Faculty of Biotechnology, University of Surabaya, Surabaya
  • Alfian Hendra Krisnawan Faculty of Pharmacy, University of Surabaya, Surabaya

Abstract

The success of culture initiation depends on explant surface sterilization techniques. Suitable concentration, combinations, and duration of exposure of sterilizing agents are important to raise in vitro culture successfully. The aim of this work is to obtain the suitable sterilization method for explant buds of red ginger rhizome to get the axenic culture. Four sterilizing agents, fungicide, bactericide, Cefotaxime antibiotic, and NaOCl were tested for sterilization by various concentration and duration of exposure. The results showed that sterilizing agents 200 mg/L Cefotaxime and 100 mg/L Benomyl combined with NaOCl decreased the contamination of explants, and achieved 20% axenic culture.

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Published
2020-06-18
How to Cite
HARDJO, Popy Hartatie; KRISNAWAN, Alfian Hendra. Rhizome Buds Disinfection for Preparation of Red Ginger (Zingiber officinale Roxb. var. rubrum Rosc.) In Vitro Culture. MPI (Media Pharmaceutica Indonesiana), [S.l.], v. 3, n. 1, p. 19-26, june 2020. ISSN 2527-9017. Available at: <https://journal.ubaya.ac.id/index.php/MPI/article/view/2836>. Date accessed: 20 sep. 2020. doi: https://doi.org/10.24123/mpi.v3i1.2836.
Section
Original Research Articles