Limit of Detection Test on Salmonella spp testing on Processed Food Products Egg Pindang According to ISO 16140-3: 2021
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Abstract
Abstract—The development of pathogenic bacteria detection methods in food products has led to the emergence of faster, shorter, and more efficient techniques. However, when choosing a reference method for testing, it is crucial to ensure its reliability. The purpose of this study was to determine the limit of detection (LOD) for Salmonella testing in processed chicken egg food products (Pindang Egg). The research aims to provide valuable information and serve as a reference for similar studies. The method used in this study follows ISO 6579-1:2017, while the detection limit is determined according to ISO 16140-3:2021. The results from observations on pre-enrichment and enrichment media showed that all inoculated samples exhibited a color change to cloudy, indicating bacterial growth in the media. Isolation on selective media and conformational tests confirmed 100% positivity for all samples in contamination variations of 11, 3.67, and 1.22. However, for contamination variation of 0.41, only 75% of the analyzed test data detected the presence of Salmonella, with 1 out of 4 replications not detecting it (approximately 20% failure rate). In conclusion, the lowest LOD value that can be reliably detected is 100% in the contamination variation of 1.22, while for the 0.41 variation, the detection rate only reaches 75% of the analyzed test data.
Keywords: bacteria, eggs, pathogen, salmonella
Abstrak—Pengembangan metode deteksi bakteri patogen dalam produk pangan telah menghasilkan teknik yang lebih cepat, lebih singkat, dan lebih efisien. Namun, saat memilih metode referensi untuk pengujian, sangat penting untuk memastikan kehandalannya. Tujuan dari penelitian ini adalah untuk menentukan batas deteksi (LOD) untuk pengujian Salmonella dalam produk pangan telur ayam olahan (Pindang Egg). Penelitian ini bertujuan untuk memberikan informasi berharga dan menjadi referensi untuk penelitian serupa. Metode yang digunakan dalam penelitian ini mengikuti ISO 6579-1:2017, sementara batas deteksinya ditentukan sesuai dengan ISO 16140-3:2021. Hasil pengamatan pada media pra-pemupukan dan pemupukan menunjukkan bahwa semua sampel yang diinokulasi menunjukkan perubahan warna menjadi keruh, menandakan pertumbuhan bakteri dalam media. Isolasi pada media selektif dan uji konformasi mengkonfirmasi positivitas 100% untuk semua sampel dalam variasi kontaminasi sebesar 11, 3,67, dan 1,22. Namun, untuk variasi kontaminasi sebesar 0,41, hanya 75% data uji yang dianalisis mendeteksi keberadaan Salmonella, dengan 1 dari 4 replikasi tidak mendeteksinya (sekitar 20% tingkat kegagalan). Sebagai kesimpulan, nilai LOD terendah yang dapat dideteksi dengan handal adalah 100% dalam variasi kontaminasi sebesar 1,22, sementara untuk variasi 0,41, tingkat deteksinya hanya mencapai 75% dari data uji yang dianalisis.
Kata kunci: bakteri, telur, patogen, salmonella
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References
British Standard. 2020. Microbiology of the food chain. Horizontal method for the detection, enumeration and serotyping of Salmonella. Part 1, Detection of Salmonella spp. London: British Standards Institution.
British Standard. 2021. Microbiology of the food chain. Method validation Protocol for the verification of reference methods and validated alternative methods in a single laboratory, Definitive. British Standards Institution.
Carlin, C. R., Lau, S. S., Cheng, R. A., Buehler, A. J., Kassaify, Z., & Wiedmann, M. (2020). Validation Using Diverse, Difficult-to-Detect Salmonella Strains and a Dark Chocolate Matrix Highlights the Critical Role of Strain Selection for Evaluation of Simplified, Rapid PCR-Based Methods Offering Next-Day Time to Results. Journal of Food Protection, 83(8), 1374–1386. https://doi.org/10.4315/jfp-20-066
Coelho, L., Melo, R., Monteiro, G., Reischak, D., Mendonça, A., Tavares, A., & Rossi, D. (2021). Validation of Alternative Methods of Detection of Salmonella Spp. in Experimentally Contaminated Poultry Environmental Samples. Brazilian Journal of Poultry Science, 23(2). https://doi.org/10.1590/1806-9061-2020-1380
Hantash, T., Nauta, M., Al-Sabi, M. N. S., Al-ali, W. Q., & Vigre, H. (2020). The “Rapid’Salmonella” Method: Estimation of the Limit of Detection for Salmonella Strains Typhimurium and Enteritidis Isolated from Frozen Poultry Meat. Modern Applied Science, 14(12), 43. https://doi.org/10.5539/mas.v14n12p43
Lins, P. (2018). Detection of Salmonella spp. in spices and herbs. Food Control, 83, 61–68. https://doi.org/10.1016/j.foodcont.2017.05.040
Löfström, C., Hansen, F., Mansdal, S., & Hoorfar, J. (2012). Detection of Salmonella in Meat: Comparative and Interlaboratory Validation of a Noncomplex and Cost-Effective Pre-PCR Protocol. Journal of AOAC INTERNATIONAL, 95(1), 100–104. https://doi.org/10.5740/jaoacint.11-093
Löfström, C., Krause, M., Josefsen, M. H., Hansen, F., & Hoorfar, J. (2009). Validation of a same-day real-time PCR method for screening of meat and carcass swabs for Salmonella. BMC Microbiology, 9(1), 85. https://doi.org/10.1186/1471-2180-9-85
Maddocks, S., Olma, T., & Chen, S. (2002). Comparison of CHROMagar Salmonella Medium and Xylose-Lysine-Desoxycholate and Salmonella-Shigella Agars for Isolation of Salmonella Strains from Stool Samples. Journal of Clinical Microbiology, 40(8), 2999–3003. https://doi.org/10.1128/jcm.40.8.2999-3003.2002
Malorny, B., Hoorfar, J., Bunge, C., & Helmuth, R. (2003). Multicenter Validation of the Analytical Accuracy of Salmonella PCR: towards an International Standard. Applied and Environmental Microbiology, 69(1), 290–296. https://doi.org/10.1128/aem.69.1.290-296.2003
Nugraha, R., Nurilmala, M., Nurjanah, & P Pratama. (2020). Detection of Salmonella sp. in fisheries product using real-time PCR. IOP Conference Series: Earth and Environmental Science, 404(1), 012012. https://doi.org/10.1088/1755-1315/404/1/012012
Nye, K. J., Fallon, D., Frodsham, D., Gee, B., Graham, C., Howe, S., Messer, S., Turner, T., & Warren, R. E. (2002). An evaluation of the performance of XLD, DCA, MLCB, and ABC agars as direct plating media for the isolation of Salmonella enterica from faeces. Journal of Clinical Pathology, 55(4), 286–288. https://doi.org/10.1136/jcp.55.4.286
Sophian, A. (2022). Escherichia coli Bacteria Test on Polluted Meatballs With Several Variations of Positive Control Concentration. BiosciED: Journal of Biological Science and Education, 3(1), 32–38. https://doi.org/10.37304/bed.v3i1.4908
Sophian, A., Purwaningsih, R., Igirisa, E. P. J., Amirullah, M. L., Lukita, B. L., & Fitri, R. A. (2021). Short Communication: Detection of Salmonella typhimurium ATCC 14028 and Listeria monocytogenes ATCC 7644 in processed meat products using Real-Time PCR Multiplex Method. Asian Journal of Natural Product Biochemistry, 19(1). https://doi.org/10.13057/biofar/f190103
Sophian, A., Purwaningsih, R., Lukita, B. L., & Ningsih, E. C. (2020). Detection of Salmonella typhimurium ATCC 14028 in supplement health product liquid preparation using Real-Time PCR (qPCR). Biofarmasi Journal of Natural Product Biochemistry, 18(2). https://doi.org/10.13057/biofar/f180202
Sophian, A., Purwaningsih, R., Muindar, M., Igirisa, E. P. J., & Amirullah, M. L. (2021). Detection of Salmonella typhimurium ATCC 14028 in Powder Prepared Traditional Medicines Using Real-Time PCR. Borneo Journal of Pharmacy, 4(3), 178–183. https://doi.org/10.33084/bjop.v4i3.1838
Sophian, A., Purwaningsih, R., Sutrisno, M. T., Purwadi, P., & Wahyudi, A. (2022). Detection of salmonella typhimurium bacteria on bakery products samples using boiling isolation technique. Jurnal Farmasi Sains Dan Praktis, 274–280. https://doi.org/10.31603/pharmacy.v8i3.5550
Sophian, A., Utaminingsih, S., Bhakti, T. S., & Rahmawati, D. (2023). Real-time PCR application in confirmation test of Salmonella Typhimurium on instant noodle. HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY, 13(1), 3–9. https://doi.org/10.46223/hcmcoujs.tech.en.13.1.2479.2023
Sturza, R., Mitin, V., Mitina, I., Buga, A., Zgardan, D., & Behta, E. (2021). Development and Validation of SYBR Green-Based qPCR Technique of Detection and Quantification of Salmonella enterica. Food and Nutrition Sciences, 12(11), 997–1007. https://doi.org/10.4236/fns.2021.1211073
Taskila, S., Tuomola, M., & Ojamo, H. (2012). Enrichment cultivation in detection of food-borne Salmonella. Food Control, 26(2), 369–377. https://doi.org/10.1016/j.foodcont.2012.01.043
Zhao, L., Wang, J., Sun, X. X., Wang, J., Chen, Z., Xu, X., Dong, M., Guo, Y., Wang, Y., Chen, P., Gao, W., & Geng, Y. (2021). Development and Evaluation of the Rapid and Sensitive RPA Assays for Specific Detection of Salmonella spp. in Food Samples. Frontiers in Cellular and Infection Microbiology, 11. https://doi.org/10.3389/fcimb.2021.631921.
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